产品特性

- 20分钟内快速提取微生物纯培养的DNA

- 获得大分子DNA片段可达50kb，可直接用于PCR、限制性内切酶消化等下游实验

- 可提取真菌、酵母、格兰性阴阳性菌的DNA

- 不使用对人体有害的有机溶剂，无需使用酶

描述

  UltraClean^® Microbial DNA Isolation kit专门设计用于提取1.8mL细菌纯培养高质量基因组DNA。经测试能成功提取各种微生物包括真菌孢子、酵母。

  首先，微生物在加热、去垢剂和机械联合作用下崩解，释放的DNA吸附到硅胶离心柱上，经过清洗洗脱重悬于DNA-free的Tris缓冲液中。推荐使用我们的涡旋仪及适配器，以达到更佳的提取效果。

 研究与深入

UltraClean^® Microbial DNA Isolation Kit
提取的各种不同微生物的DNA

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条带1:1kb ladder 条带2： E. coli 条带3： P. aeruginosa 条带4： S. aureus 条带5： M. luteus 条带6： B. subtilis 条带7： B. pumulis

条带8： E. faecalis 条带9： L. casei 条带10： 1 kb ladder 条带11： A. niger 条带12： C. albicans 条带13： S. cerevisiae 条带14： S. cerevisiae

1.2% TAE琼脂凝胶电泳图。A. niger (11) 和 C. albicans (12)
纯培养细菌拷贝数低，出来条带浓度小。

规格

方法	离心柱型
细胞破碎	研磨珠破碎
滤膜吸附能力	20 µg每孔
通量	1 - 24 个样
操作时间	20 分钟

使用 UltraClean® Kit 测试过的微生物类型
B. subtilis B. pumulis E. coli E. faecalis P. aeruginosa M. luteus	S. aureus L. casei A. niger C. albicans S. cerevisiae

 

保存条件

室温保存2年。

文献

• Beare, P.A., Unsworth, N., Andoh, M., Voth, D.E., Omsland, A., Gilk, S.D., Williams, K.P., Sobral, B.W., Kupko III, J.J., Porcella, S.F., Samuel, J.E., and Heinzen, R.A. Comparative Genomics Reveal Extensive Transposon-Mediated Genomic Plasticity and Diversity amoing Potential Effector Proteins within the Genus Coxiella. Infection and Immunity. 77(2): 642-656 (2009). 
• Chen, Y., Zhang, W., and Knabel, S.J. Multi-Virulence-Locus Sequence Typing Clarifies Epidemiology of Recent Listeriosis Outbreaks in the United States. Journal of Clinical Microbiology. 43(10): 5291-5294 (2009).
• Dauphin, L.A., Moser, B.D., and Bowen, M.D. Evaluation of Five Commercial Nucleic Acid Extraction Kits for their Ability to Inactive Bacillus anthracis Spores and Comparison of DNA Yields from Spores and Spiked Environmental Samples. Journal of Microbiological Methods. 76(1): 30-37 (2009).

客户评价

New Method for Ectomycorrhizal Roots

The MO BIO UltraClean^® Microbial DNA Kit works well for us. We have been trying to isolate DNA from ectomycorrhizal roots. Published methods for this are available, but they are slow and may involve highly toxic chemicals. When isolating DNA from pine ectomycorrhizas, we use the Microbial DNA Kit in conjunction with IRS (Inhibitor Removal Solution, available from MO BIO) and obtain PCR-ready DNA. No grinding or freezing is necessary. The process is simple and rapid.

Roger Koide
宾西法利亚州立大学The Pennsylvania State University

试剂盒组分

组分	12224-50
Solution MD1 Solution MD2 Solution MD3 Solution MD4 Solution MD5 Microbead Solution Microbead Tubes Spin Filters In Tubes Collection Tubes	1 x 2.75 ml 1 x 5.5 ml 1 x 50 ml 1 x 16.5 ml 1 x 3 ml 50 1 x 16.5 ml 50 200