产品特性

- 6分钟快速提取高质量质粒DNA

- 采用了硅胶离心柱法

- 1-2ml菌液提取高达20μg 质粒

- 低拷贝数细菌推荐使用TB DRY^® Powder Growth Media培养（货号：12105）

- 试剂盒包含所有需要的试剂与Tube管

描述

  UltraClean^® 6 Minute Mini Plasmid Prep Kit提供了整套所需试剂非常快速地提取1-5ml菌液的质粒DNA。使用了离心柱，更终得到的DNA可浓缩到50~100μl。看！6分钟提取质粒DNA就如此简单！获得的高质量DNA可直接用于测序、酶切、连接、PCR等下游实验。想用传统的方法提取。
  这个试剂盒有个独特的优点在于能处理高营养培养基。高营养培养基可改善低拷贝数，而大部分商业化试剂盒处理高营养培养基效果不理想，往往带入基因组DNA。UltraClean^® 6 Minute Mini Plasmid Prep Kit在从低拷贝数质粒中获得高得率同时，只需要6分钟就能完成操作。

 研究与深入

  使用MoBio实验室的Ultra Clean® 6 Minute Mini Plasmid Prep Kit与竞争厂家的产品同时提取过夜的2mL LB 培养菌液的质粒DNA。

  电泳结果表明两家结果无区别。

  A260/280 及 A260/230 比值表明质粒DNA纯度差不多。

  应用PicoGreen发定量dsDNA浓度，显示两者之间无差异。

规格

方法	硅胶离心柱型
细胞裂解	碱性裂解液裂解
滤膜DNA吸附能力	20 µg每孔
通量	1 - 24 个样
操作时间	6 分钟
仪器设备	微型离心机

保存条件

室温保存2年。

文献

• Caliante, S., Prisco, M., Sciarrillo, R., De Falco, M., Capaldo, A., Gay, G., Andreuccetti, P., Laforgia, V., and Varano, L.Pituitary adenylate cyclase-activating polypeptide, vasoactive intestinal polypeptide and their receptors: distribution and involvement in the secretion of Podarcis sicula adrenal gland . Journal of Endocrinology. 196 291-303 (2008).
• Lopez-Matas, M.A., Nuñez, P., Soto, A., Allona, I., Casado, R., Collada, C., Guevara, M.A., Aragoncillo, C., Gomez, L. Protein Cryoprotective Activity Of a Cytosolic Small Heat Shock Protein That Accumulates Constitutively In Chestnut Stems and Is Up-Regulated By Low and High Temperatures. Plant Physiology. 134(4): 1708-1717 (2004).
• Scott, H.N., Laible, P.D., Hanson, D.K. Sequences Of Versatile Broad-Host-Range Vectors Of The RK2 Family. Plasmid. 50(1): 74-79 (2003).

客户评价

Excellent Product
"I was very impressed with the MO BIO UltraClean^® 6 Minute Mini Plasmid Prep Kit. I have used standard mini prep protocols and kits from other companies with variable results. The MO BIO kit gave clean, sequence ready DNA. The kit was user friendly, economical and fast. Less than $0.05/ug of clean plasmid DNA means it it less expensive than other leading brands and supplies plenty of plasmid for restriction digests, sequencing and other applications. Nice product."

fowlkes@reed.edu

UltraClean^® 6 Minute Mini Plasmid Prep Kit
"I liked the kit. It is very fast, easy and simple to use. Plasmid was of good quality, worked for restriction digestion as well as transfections."

krupas@ncifcrf.gov
 

试剂盒组分

组分	12300-50
Solution 1 Solution 2 Solution 3 Solution 4 Solution 5 Tubes Spin Filters	1 x 3.3 ml 1 x 5.5 ml 1 x 18 ml 1 x 16.5 ml 1 x 3.3 ml 100 50