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| 美国MOBIO 强力土壤DNA提取试剂盒(PowerSoil® DNA Isolation Kit) |
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PowerSoil® DNA Isolation Kit
OverviewFor isolating genomic microbial DNA of the highest quality and purity from most environmental samples. Ideally suited for all microbial soil sample collections.
Order
| Description |
Catalog # |
Price |
| 50 Preps |
12888-50 |
N/A |
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| 100 preps |
12888-100 |
N/A |
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Features
- Next generation method improves PCR inhibitor removal efficiency
- Processes all soil types, including difficult environmental samples
- Patented technology removes 100% of humic substances and PCR inhibitors
- Rapid protocol enables purification of DNA from 250 mg soil in 30 minutes
- Applications include biodefense, soil-borne pathogen detection, agriculture and zoology research
Description
The PowerSoil® DNA Isolation Kit is our next generation soil DNA kit, which uses our novel, patented Inhibitor Removal Technology® to extract microbial DNA from all soil types and other environmental samples. The isolated DNA has the highest level of purity allowing for more successful PCR and QPCR amplification of organisms from the sample. The kit is ideal for processing all environmental samples including difficult types containing a high humic acid content such as compost, sediment, and manure. PCR analysis has been performed to detect a variety of organisms including both Gram-positive and Gram-negative bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae, and actinomycetes (e.g. Streptomyces), and nematodes.
The PowerSoil® DNA Isolation Kit distinguishes itself from other kits with a patented humic substance/brown color removal procedure that removes PCR inhibitors from even the most difficult soil types, promoting successful downstream PCR analysis. When choosing between the PowerSoil® DNA Isolation Kit and the UltraClean® Soil DNA Isolation Kit, use this kit for samples that are very high in humic content.
Do not let the name fool you. Our customers use this kit successfully on diverse sample types such as food (cheese, milk, meat, fruit), water, insects and fecal samples in a variety of applications including biodefense, agriculture and zoology. These soil kits are used by the USEPA, USDA, FBI and CDC for soil analysis.
NEW: Speed your protocol up to 20% in using our PowerVac™ Manifold Mini System!
Sample Kit
Click to try a sample.
Research and Development
The PowerSoil® DNA Isolation Kit is More Effective for Community Analysis by PCR
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High Yield, Intact Genomic DNA |
MO BIO
PowerSoil®
Supplier A
Supplier B |
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M 1 2 3 4 5 6 7 8 9 |
Total Genomic DNA was isolated from nine different
sample types using the MO BIO PowerSoil® DNA Isolation Kit
and soil DNA isolation kits from two other suppliers. All
isolations were performed following the manufacturerís
protocols. Eluted genomic DNA was displayed on a 0.8%
TAE agarose gel (15 µl per lane).
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Confidence in Your PCR Analysis |
MO BIO
PowerSoil®
Supplier A
Supplier B |
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M 1 2 3 4 5 6 7 8 9 |
PCR analysis using eubacterial primers was performed
on 1 µl of the undiluted DNA eluate. PCR products
were displayed on a 0.8% TAE agarose gel.
Lane M is DNA Marker.
| Lane 1 - Landfill 0-3 inches |
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Lane 4 - Coffee Compost |
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Lane 7 - Mud Sediment |
| Lane 2 - Landfill 3-6 inches |
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Lane 5 - Marine Sediment |
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Lane 8 - Horse Manure |
| Lane 3 - Late-stage Compost |
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Lane 6 - Lake Sediment |
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Lane 9 - Mulch Topsoil |
Only DNA isolated with PowerSoil® yielded a PCR product for Landfill samples (lanes 1 and 2), Lake Sediment (lane 6) and Mulch Topsoil (lane 9). DNA isolated using other supplier kits failed to amplify products for these samples, most likely due to high levels of humic acid substances remaining in the final DNA eluate.
Positive PCR amplification using PowerSoil® was observed with 100% of the samples. PCR analysis resulted in only 44% positive amplification with supplier kits tested.
 Click over image to enlarge
Specifications
| Format |
Silica Spin Filter Tubes |
| Method |
Bead Beating |
| Binding Capacity |
Up to 20 µg per filter |
| Sample Size |
250 mg |
| Throughput |
1 - 24 samples |
| Time |
30 minutes |
| Equipment Required |
Vortex and Vortex Adapter |
Storage ConditionsStore at room temperature for up to 2 years.
Publications
- Alcantara-Hernandez, R.J., Valenzuela-Encinas, C., Marsch, R., and Dendooven, L. Respiratory and Dissimilatory Nitrate-reducing Communities from and Extreme Saline Alkaline Soil of the Former Lake Texcoco (Mexico). Extremophiles. 13(1): 169-178 (2009).
- Foster, J.S., Green, S.J., Ahrendt, S.R., Golubic, S., Reid, R.P., Jetherington, K.L., and Bebout, L. Molecular and Morphological Characterization of Cyanobacterial Diversity in the Stormatolites of Highborne Cay, Bahamas. ISME Journal. 1-15 (2009).
- Giubilei, M.A., Leonardi, V., Federici, E., Covino, S., Sasek, V., Novotny, C, Federici, F., D’Annibale, A., and Petruccioli, M. Effect of Mobilizing Agents on Mycoremediation and Impact on the Indigenous Microbiota. Chemical Technology and Biotechnology. 84(6): 836-844 (2009).
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