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美国MOBIO 96孔土壤DNA提取试剂盒(UltraClean®-htp 96 Well Soil DNA Isolation Kit)

 

 

UltraClean®-htp 96 Well Soil DNA Isolation Kit

Overview

For high throughput isolation of DNA from up to 384 soil samples in less than one day
Order
Description Catalog # Price
4 x 96 preps 12896-4 N/A
12 x 96 preps 12896-12 N/A

Features

- Processes up to 384 soil samples in less than one day.

- Yields high quality PCR ready DNA up to 50kb plus

- Includes Inhibitor Removal Technology® to eliminate humic acid inbitors of PCR

- Efficiently lyses spores and fungi; also compatible with fecal samples

- Flexible format with protocols available for centrifiguation or vacuum

Description

The UltraClean®-htp 96 Well Soil DNA Isolation Kit provides the UltraClean® soil technology in a high-throughput 96 well format. This kit requires a plate centrifuge, a 96 well plate shaker and optional Vacuum Manifold.
The UltraClean®-htp 96 Well Soil DNA Isolation Kit has become the method of choice among researchers around the world studying microbial organisms in soil. This kit will isolate cellular, PCR quality DNA from soil and ensures removal of humic acid inhibitors.

Soil samples are added to a 96 well block containing beads, lysis solution, bead solution and Inhibitor Removal Solution. The principal is to lyse the microorganisms in the soil by a combination of heat, detergent, and mechanical force against specialized beads. The cellular components are lysed by mechanical action on a 96 well plate shaker. From the lysed cells, the released DNA is bound to a silica spin plate. The plate is washed, and the DNA is recovered in certified DNA-free Tris buffer.
 

Research and Development

Consistent Yields and
Well-to-Well Reproducibility obtained with
UltraClean®-htp 96 Well Soil DNA Isolation Kit
 

 


Genomic DNA was isolated from different soil samples.
Analysis displayed on 1.2% TAE agarose gel.

Learn more about the applications of the
UltraClean®-htp 96 Well Soil DNA Isolation Kit in this article.

Novel High Throughput Method for Studying
Microbial Communities and Genes in Soil


Download Here (pdf 1.8MB)

 

 

UltraClean®-htp 96 Well Soil DNA Isolation Kit
Click over image to enlarge

MSDS

12896

Protocol

12896

Specifications

Format Silica Spin FilterPlate
Method Bead Beating
Binding Capacity Up to 20 µg per well
Sample Size 250 mg
Throughput 96 - 384 samples
Time 4 x 96 samples in <1 day

Equipment Required
Plate centrifuge, 96 well plate
shaker, UltraVac™ Manifold*

* Optional

Positive PCR Results
Obtained With
These Soil Types
  • Clay
  • Fecal/Intestinal Tract
  • Fungal mat
  • Forest
  • Loam
  • Sand
  • Potting Soil
  • Petroleum-Saturated
  • Aquifer Sediment

Storage Conditions

Store at room temperature for up to 2 years.

Publications

Kit Components

Component 12896-4 12896-12
Bead Plates
Bead Solution
Solution S1
IRS Solution
Solution S2
Solution S3
Solution S4
Solution S5
Spin Plates
2 ml Collection Plates
1 ml Collection Plates
0.5 ml Collection Plates
Microplates
Centrifuge Tape
Sealing Tape
Elution Sealing Mat
4
1 x 233 ml
1 x 26 ml
1 x 85 ml
1 x 106 ml
1 x 550 ml
4 x 30 ml
1 x 43 ml
4
8
8
4
4
24
8
4
12
1 x 699 ml
1 x 78 ml
1 x 255 ml
1 x 318 ml
1 x 1650 ml
12 x 30 ml
1 x 129 ml
12
24
24
12
12
72
24
12

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