产品特性
- 45分钟快速提取高质量质粒DNA
- 获得的高质量DNA可直接进行酶切、测序、标记或PCR
- 采用了大容量硅胶离心柱
- 从标准培养或高营养培养基都能获得大量DNA
描述
UltraClean® Maxi Plasmid Prep Kit专门设计用于提取大体积
E. coli菌液的质粒DNA。与UltraClean® 6 Minute Mini Plasmid Prep Kit同样使用了硅胶离心柱。可以处理500ml LB培养基或250ml高营养培养基。
先由改进配方的碱性裂解液裂解细菌，DNA吸附到硅胶离心柱滤膜上，经洗涤重悬与小体积的缓冲液中。更终DNA浓度较大，可直接进行自动测序，或进行酶切、标记、PCR等下游使用。
研究与深入
一致的高得率
条带1：Marker
条带2-5：UltraClean® Maxi Plasmid Prep Kit
提取了的几个菌液的pUC19 |
规格
| 方法 |
50 ml硅胶离心柱 |
| 细胞破碎 |
碱性裂解液裂解 |
| 滤膜DNA吸附能力 |
1mg每孔 |
菌液量
|
TB DRY®, 250 ml
LB Broth, 500 ml |
| 通量 |
1 - 4 个样 |
| 操作时间 |
45 分钟 |
保存条件室温保存2年。
文献
- Vassilopoulos, A., Gaitanaki, C., Papazafiri, P., Beis, I. Atrial Natriuretic Peptide mRNA Regulation by p38-MAPK in the Perfused Amphibian Heart . Cellular Physiology and Biochemisty. 16: 183-192 (2005).
- Walker, T.L., Becker, D.K., Collet, C. Characterisation of the Dunaliella tertiolecta RbcS Genes and Their Promoter Activity in Chlamydomonas reinhardtii. . Plant Cell Reports. 23: 727-735 (2005).
- Stevens, T.A., Iacovoni, J.S., Edelman, D.B., Meech, R. Identification Of Novel Binding Elements and Gene Targets For The Homeodomain Protein BARX2. Journal of Biological Chemistry. 279(15): 14520-14530 (2004).
客户评价
Best Value for the Money; Best Yield for the Time.
After using other maxiprep kits, including those from Clontech, Qiagen, Sigma and BioRad (both the Quantum Prep and the new Aurum) kits, as well as doing the protocol straight from Sambrook, I can only say that your kit gives me the best, purest, highest yield per dollar in the least amount of time. Sure, the package is not as pretty as the other kits, but as a very busy research associate/lab mommy I just do not have time to be making maxipreps time after time or to spend two or three days completing them. I do not have time to spend on additional purifications to get a "glassy" pellet that actually goes into solution properly. Your kit is the best kit for my needs.
Lisa Miller
University of Nevada Reno
Great, Clean DNA.
This was my first time using a MO BIO product. I needed to generate a relatively large amount of a low copy plasmid. I used the MO BIO TB DRY® Powder Growth Media to grow 250 ml of E.coli containing my plasmid. Then I used the maxi plasmid kit and generated approximately 80 µg of my low copy plasmid. This was an excellent yield for this construct. The DNA was sent out for sequencing and so far our results from that are beautiful also. I strongly recommend this kit for all ultra-clean plasmid preps.
hscott@anl.gov
试剂盒组分
| 组分 |
12600-10 |
12600-20 |
Solution 1
Solution 2
Solution 3
Solution 4
Solution 5
Spin Filters in Tubes
50 ml Tubes |
1 x 52.5 ml
1 x 105 ml
1 x 315 ml
1 x 110 ml
1 x 45 ml
10
20 |
1 x 105 ml
1 x 210 ml
2 x 315 ml
1 x 210 ml
1 x 88 ml
20
40 | |
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