DESCRIPTION
The ZymoBIOMICS RNA Miniprep Kit is designed for purifying RNA from a wide array of sample inputs that is ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae). The procedure uses Zymo-Spin Column technology that results in high-quality total RNA (including small RNAs 17-200 nt) that is free of PCR inhibitors and is ready for RT-PCR, hybridization, sequencing, etc. DNase I included.
HIGHLIGHTS
- Unbiased Lysis: Efficient and unbiased lysis of microbes including Grampositive/negative bacteria, fungi, protozoans, and viruses from any sample (feces, soil, plant, water, biofilms, swabs, saliva, body fluids, etc.)
- Ultra-Pure: Total RNA (including small/micro RNAs) is inhibitor-free and ready for qPCR and microbiome measurements using Next-Gen Sequencing.
- High Sensitivity: Increased detection limit of very low abundance organisms.
TECHNICAL SPECIFICATIONS
| Equipment |
Microcentrifuge, vortex, cell disrupter (recommended) |
| Purity |
RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8. |
| Sample Source |
Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host RNA is efficiently isolated from ≤ 200 mg of mammalian feces, ≤ 250 mg soil, ≤ 200 mg plant/seed, 50-100 mg (wet weight) fungal bacterial cells, biofilms, and water. |
| Size Range |
Total RNA ≥17 nt |
| Yield |
100 µg RNA (binding capacity), ≥50 µl (elution volume) |
| Cat # |
Name |
Size |
| R2001 |
ZymoBIOMICS RNA Miniprep Kit |
50 Preps |
|
