DESCRIPTION
The ZR small-RNA PAGE Recovery Kit provides an easy and efficient method for the extraction of high quality small RNAs from polyacrylamide gels (native and/or denatured). The ZR small-RNA™ PAGE Recovery Kit is a refinement of the "crush and soak" method that incorporates a unique buffer system together with Zymo-Spin column technologies for improved recovery and added convenience. The recovered RNA can be concentrated into volumes as small as 6 µl, making it ideal for many downstream enzymatic reactions and manipulations.
HIGHLIGHTS
- Efficient: Recovery of ss- or ds- RNA (and DNA) fragments (17-200 nt) from up to 20% (w/v) polyacrylamide gels.
- Concentrated: Up to 10 µg sample in ≥ 6 µl elution.
- Compatible with up to 25% (w/v) polyacrylamide.
TECHNICAL SPECIFICATIONS
| Equipment |
Microcentrifuge, 37°C-65°C heat source, dry ice or -80°C freezer |
| Purity |
RNA is ready for all subsequent analysis and molecular manipulation |
| Sample Source |
RNA or DNA fragments (17-200 nt) resolved in PAGE gels (up to 25%), also compatible with TBE-, denatured, urea PAGE gels. Compatible with ethidium bromide or ssRNA-specific dyes (e.g. GelStar). |
| Size Range |
RNAs 17-200 nt (fragments 17-28 nt is ≥50 % recovery) |
| Yield |
10 µg RNA (binding capacity), ≥6 µl (elution volume) |
| Cat # |
Name |
Size |
| R1070 |
ZR small-RNA PAGE Recovery Kit |
20 Preps |
|
