DESCRIPTION
The Zymoclean Gel RNA Recovery Kit provides a quick and efficient purification method for recovery of RNA fragments from agarose gels. The procedure combines a unique, single-step agarose dissolving/RNA binding buffer with Zymo-Spin column technology to yield high quality, purified RNA in just minutes. The purified RNA is eluted into small volumes of DNase/RNase-free water for highly concentrated samples suitable for subsequent RNA-based manipulations. Compatible with MOPS, TAE, and TBE buffered agarose gels (formaldehyde up to 2.0%).
HIGHLIGHTS
- Quick and Reliable: 30 minute recovery of purified RNA fragments from agarose gels.
- Concentrated: Up to 10 µg sample in ≥ 6 µl elution.
- High Recovery: ≥ 80% recovery for RNA > 500 nt.
TECHNICAL SPECIFICATIONS
| Equipment |
Microcentrifuge, 37°C-65°C heat source |
| Purity |
RNA is ready for all subsequent analysis and molecular manipulation |
| Sample Source |
Single- or double-stranded RNA fragments (≥200 nt) resolved in TAE TBE, and MOPS buffered agarose gels. Compatible with formaldehyde to 2.0% (final conc.) |
| Size Range |
RNA fragments ≥500 nt (≥80% recovery) |
| Yield |
10 µg RNA (binding capacity), ≥6 µl (elution volume) |
| Cat # |
Name |
Size |
| R1011 |
Zymoclean Gel RNA Recovery Kit |
50 Preps |
|
