DESCRIPTION
The Quick-cfRNA Serum & Plasma Kit enables simple, reliable, and efficient preparation of high-quality circulating cell-free RNA (including protein-bound, exosomal, miRNA and other small RNAs) from serum, plasma and other biological fluids. The kit does not use toxic phenol-chloroform or cumbersome protein precipitation and removal steps. Recovered cell-free RNA scales linearly relative to the sample input volume up to 3 ml. ZymoSpin™ Technology enables isolation of ultra-pure cell-free RNA suitable for all subsequent analyses, such as RT-qPCR and NGS. Empower your discovery of circulating RNA biomarkers.
HIGHLIGHTS
- Quick & Easy: Simple spin-column based isolation from up to 3 mL of plasma, serum, or other biological liquids. No phenol/chloroform or precipitation.
- Highest Yields: Efficient isolation of total cell-free RNA. Recover up to 515 times more microRNA.
- Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, nCounter, etc.
TECHNICAL SPECIFICATIONS
| Elution Volume |
cfRNA can be eluted into 15 µl (to maximize total yield) or as little as 6 µl (for highly concentrated eluate) of DNase/RNase-Free Water. |
| Processing Time |
Typically requires 30 minutes hands-on time to process 10 samples. Sample digestion requires 2 hours. |
| Purity |
High quality cell-free RNA (cfRNA) is ready for all sensitive downstream applications such as RT-qPCR and Next-Generation Sequencing. |
| Required Equipment |
Microcentrifuge, vortex, water bath or heating block, vacuum, and vacuum manifold. |
| Sample Type |
Up to 3 ml of plasma, serum, cerebrospinal fluids, amniotic fluids, urine, or other biological fluids. |
| Size |
RNA ≥ 17 nt. |
| Yield |
Yields are sample dependent and may vary. Typical recovery ranges from 1 – 100 ng/ml of human plasma or serum. Kit is optimized for recovery of total cfRNA, including small RNA and microRNA. |
| Cat # |
Name |
Size |
| R1059 |
Quick-cfRNA Serum & Plasma Kit |
50 preps |
|
