DESCRIPTION
The Quick-RNA Whole Blood kit utilizes DNA/RNA Shield™, a unique preservation and lysis technology, to enable rapid isolation of total RNA from whole or partitioned blood or a cell pellet (after red blood cell lysis). The procedure uses Zymo-Spin column technology in which the sample is pre-filtered on the Zymo-Spin IIICG Column and then purified and concentrated on the Zymo-Spin IC Column. RNA is eluted into ≥ 6 µl of RNase-free water and is ready for any downstream application including RT-PCR, sequencing, etc.
HIGHLIGHTS
- Superior Yields: Recover total RNA (including small/micro RNAs) without sample loss.
- Protection: Worry-free blood sample storage at ambient temperatures for up to 30 days.
- High-Quality: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-PCR, etc. DNase I Included.
TECHNICAL SPECIFICATIONS
| Equipment |
Microcentrifuge, vortex |
| Purity |
RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8. |
| Sample Source |
Up to 1 ml mammalian whole-blood, plasma, or serum. Also compatible with pelleted blood cells (PBMCs, WBCs, buffy coat, pelleted sample from PAXgene™ Blood RNA Tube, etc.) and nucleated blood. |
| Size Range |
Total RNA ≥ 17 nt |
| Yield |
10 µg RNA (binding capacity), ≥6 µl (elution volume) |
| Cat # |
Name |
Size |
| R1201 |
Quick-RNA Whole Blood |
50 preps | |
