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Quick-cfDNA Serum & Plasma Kit provides a simple and reliable method for the rapid preparation of high-quality circulating cell-free DNA from serum, plasma, amniotic fluid, cerebrospinal fluid (CSF), and saliva. A combination of chemical and enzymatic methods are used to efficiently recover total DNA (including cell-free apoptotic, necrotic, mitochondrial and viral DNA) linearly from up to 10 ml of sample (Reference Figure A). Zymo-Spin technology allows for ultra-pure DNA to be eluted in as little as 35 μl water. The resulting DNA is suitable for all subsequent analyses and molecular manipulations such as qPCR, Next-Generation sequencing and DNA methylation analyses. Zymo’s serum and plasma cell-free DNA extraction technology will empower your discovery of circulating DNA biomarkers.
HIGHLIGHTS
- High Processing Volume: Purify ≤ 10 ml of serum or plasma and elute with 35 µl.
- Highest Yields: Consistently purify > 30% more cfDNA.
- Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, etc.
| DNA Purity |
High-quality DNA is ready for all sensitive downstream applications such as qPCR and Next-Generation sequencing. |
| DNA Recovery |
Recover DNA ≥ 100bp (optimized for recovery of cell-free DNA). Yields can vary considerably among different individuals. Typically DNA recovery ranges from 1-100 ng/ml of plasma or serum. The yield varies depending on the sample source and the health of the donor. |
| DNA Storage |
Eluted DNA should be stored at ≤ -20ºC |
| Processing volume |
Plasma – Single centrifugation: up to 3 ml
Plasma – Double centrifugation: up to 10 ml
Serum – up to 10 ml
Amniotic fluid – up to 1 m
l Cerebrospinal fluid (CSF) – up to 1 ml
Saliva – up to 1 ml
Cell-free saliva – up to 5 ml |
| Recovery Volume |
≥ 35 µl of DNA Elution Buffer or DNase free water. |
| Required Equipment |
Water bath or heat block (55ºC), microcentrifuge, vacuum/vacuum manifold or swinging bucket centrifuge. |
| Sample Sources |
Serum, plasma, amniotic fluid, and cerebrospinal fluid (CSF). |
| Cat # |
Name |
Size |
| D4076 |
Quick-cfDNA Serum & Plasma Kit |
50 Preps |
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